Additional file 2 of Intracerebral overexpression of miR-669c is protective in mouse ischemic stroke model by targeting MyD88 and inducing alternative microglial/macrophage activation

Additional file 2: Supplementary Figure 2. CD45 expression does not change under miR-669c overexpression in conditions of brain ischemia. The ipsilateral CD45 immunoreactivity remained unaltered between stroke control LV1-GFP (GFP) and LV1-miR-669c mice (669) (A). Panels B-E are representative photographs of coronal sections stained with CD45 in LV1-GFP control (B, D) and LV1-miR-669c injected tMCAo animals (C, E). Similarly, the ratio of Arg1+ to round in shape, bright CD45+ cells was not changed in LV1-miR-669c animals (669) comparing to the control group (GFP) (F). Panels G-N consists of confocal microphotographs illustrating the proportion of Arg1+ and CD45+ cells in the ipsilateral striatum of LV1-GFP control (G-J) and LV1-miR-669c (K-N) stroke animals. Unpaired two-tailed t-tests. N = 6 animals per each group.