Rapid quantification of isoflurane in anesthetic nanoemulsions using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)

There is increased research into pharmaceutical nanoemulsions where the timing of quantification of active components can dictate continuous manufacturing production cost and consistency. The goal of this study was development of a rapid quantification method for isoflurane nanoemulsions using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR). Isoflurane was quantified by ATR-FTIR and High-Performance Liquid Chromatography (HPLC). Correlation and agreement between methods was determined to validate the ATR-FTIR procedure. Evaporation rate studies from the nanoemulsions and pure isoflurane were performed. ATR-FTIR values were in agreement and correlated with (HPLC) measurements (calculated Pearson R of 0.99; 99 % confidence interval; P