Isolation and Purification of Phosphatidylcholine from Egg Yolk by Semipreparative Hydrophilic Interaction Liquid Chromatography

Crude phosphatidylcholine (PC) was extracted with ethanol from egg yolk, and an L9(34) orthogonal array experimental design was set up to optimize the solvent extraction conditions. Subsequently, semipreparative hydrophilic interaction liquid chromatography (HILIC) was explored to purify the crude extract on a homemade silica column. The purity of PC prepared in this way can reach up to 93.96%, with rapid separation.