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Enantioselective adsorption of dipeptides on chiral stationary phases with grafted macrocyclic antibiotics using glycylaspartic acid as an example

Authors :
A. N. Vasyanin
Leonid Asnin
M. V. Stepanova
Source :
Russian Chemical Bulletin. 68:2232-2240
Publication Year :
2019
Publisher :
Springer Science and Business Media LLC, 2019.

Abstract

The chromatographic behavior and adsorption of enantiomers of glycylaspartic acid on the Nautilus-E chiral stationary phase with grafted eremomycin antibiotic were studied. Acetate buf er mixtures with variable pH prepared in a water—methanol mixed solvent (volume ratio 60 : 40) were used as acetate buf ers. The retention of Gly—Asp was found to be enantioselective and depending on the ratio of various ionic forms of dipeptide in the mobile phase. The ratio is determined by the pH. A model of adsorption equilibrium assuming independent binding of diverse ionic forms with the stationary phase, which is characteristic of energy uniformity, was proposed. This model is well consistent with the experimental data. Features of application of the equilibrium dispersion and transport dispersion dynamic elution models for the determination of the adsorption isotherms by the inverse method are discussed.

Details

ISSN :
15739171 and 10665285
Volume :
68
Database :
OpenAIRE
Journal :
Russian Chemical Bulletin
Accession number :
edsair.doi...........28b82b70b0e5ba2ee1764dd5662e9a8f
Full Text :
https://doi.org/10.1007/s11172-019-2692-z