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Erratum:In vitroandin vivoanti-Helicobacter pyloriactivities of FEMY-R7 composed of fucoidan and evening primrose extract
- Source :
- Laboratory Animal Research. 31:99
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Effects of FEMY-R7, composed of fucoidan and evening primrose extract, on the bacterial growth and intragastric infection of Helicobacter pylori as well as gastric secretion were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1×108 CFU/mL) was incubated with a serially-diluted FEMY-R7 for 3 days. As a result, FEMY-R7 fully inhibited the bacterial growth at 100 µg/mL, which was determined to be a minimal inhibitory concentration. In addition, 6-hour incubation with H. pylori, FEMY-R7 inhibited urease activity in a concentration-dependent manner, showing a median inhibitory concentration of 1,500 µg/mL. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (5×109 CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with 10, 30 or 100 mg/kg FEMY-R7 for 7 days. In Campylobcter-like organism-detection test and bacterial identification, FEMY-R7 exerted a high bacteria-eliminating capacity at 30-100 mg/kg, comparably to 30 mg/kg pantoprazole. In contrast to a strong antacid activity of pantoprazole in a pylorus-ligation study, FEMY-R7 did not significantly affect gastric pH, free HCl, and total acidity, although it significantly decreased fluid volume at a low dose (10 mg/kg). The results indicate that FEMY-R7 eliminate H. pylori from gastric mucosa by directly killing the bacteria and preventing their adhesion and invasion, rather than by inhibiting gastric secretion or mucosal damage.
- Subjects :
- biology
Fucoidan
business.industry
medicine.medical_treatment
Helicobacter pylori
Pharmacology
biology.organism_classification
chemistry.chemical_compound
Minimum inhibitory concentration
medicine.anatomical_structure
chemistry
In vivo
Antacid
Immunology
Gastric mucosa
medicine
business
Incubation
Pantoprazole
medicine.drug
Subjects
Details
- ISSN :
- 22337660 and 17386055
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Laboratory Animal Research
- Accession number :
- edsair.doi...........28b6fb5feb1d95145382d80703cb7691