Back to Search
Start Over
IrrE Improves Organic Solvent Tolerance and Δ1-Dehydrogenation Productivity of Arthrobacter simplex
- Source :
- Journal of Agricultural and Food Chemistry. 66:5210-5220
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- During steroid bioconversion, organic solvents are widely used for facilitating hydrophobic substrate dissolution in industry. Thus, strains that tolerate organic solvents are highly desirable. IrrE, a global transcriptional factor, was introduced into Arthrobacter simplex with Δ1-dehydrogenation ability. The results evidenced that IrrE did not affect cell biological traits and biotransformation performance under non-stress conditions. However, the recombinant strain achieved a productivity higher than that of the control strain in systems containing more ethanol and substrate, which coincided with cell viability under ethanol stress, the major stress factor during biotransformation. It also demonstrated that IrrE caused genome-wide transcriptional perturbation, and several defense proteins or systems were linked with higher organic solvent tolerance. IrrE simultaneously enhanced cell resistance to various stresses, and its horizontal impacts showed strain and stress dependence. In conclusion, the introdu...
- Subjects :
- 0106 biological sciences
0301 basic medicine
Ethanol
Bioconversion
medicine.medical_treatment
Arthrobacter simplex
General Chemistry
01 natural sciences
Steroid
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Biotransformation
010608 biotechnology
medicine
Biophysics
Dehydrogenation
Viability assay
General Agricultural and Biological Sciences
Dissolution
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi...........27d752b146c4f3a32ee201849d456290