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Design and Synthesis of BRCA1 (856-871) Analogous and their Interactions with RAD51 (158-180)
- Source :
- International Journal of Peptide Research and Therapeutics. 27:1343-1350
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Breast cancer susceptibility gene 1 (BRCA1) is an important tumor suppressor gene in the human which associated with a variety of tumor suppressor genes to inhibit the growth of malignant tumors. Breast cancer is closely related to BRCA1 mutations in humans. Four BRCA1 (856-871) mutants were screened that can interact well with RAD51 (158-180) using computer to simulate mutations at Y 856, R 866 and Q 867. These mutants were synthesized by using Fmoc solid-phase synthesis, and purified by liquid chromatography. According to the results of fluorescence spectroscopy, R866W has the strongest binding ability for the above peptides and RAD51 (158-180). Based on the circular dichroism spectrum results, the sequence of the interaction ability of the above peptides on the secondary structure of RAD51 (158-180) is Y856P, Q867C > Y856P, R866W ≈ R866W > Y856P > BRCA1. All the spectral results show that R866W have the strongest interaction ability with RAD51(158-180).
- Subjects :
- Circular dichroism
Tumor suppressor gene
010405 organic chemistry
Chemistry
Mutant
RAD51
Bioengineering
01 natural sciences
Biochemistry
Molecular medicine
Molecular biology
0104 chemical sciences
Analytical Chemistry
law.invention
law
Drug Discovery
Molecular Medicine
Suppressor
Gene
Protein secondary structure
Subjects
Details
- ISSN :
- 15733904 and 15733149
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- International Journal of Peptide Research and Therapeutics
- Accession number :
- edsair.doi...........271e6e8fcb42d1ab1817b97f398d2ca9