The development of graphene coated measuring plate for the investigation of 'dark' neurons with electrical impedance spectrum (EIS) measurement

Compacted neurons are morphologically changed axons in an undamaged environment, which are able to recover spontaneously. They may play an important role in neurological disorders, such as Parkinson’s disease or Alzheimer’s disease. The aim of this study was to find the best method for the investigation of this process. Therefore we designed and selfmade bio-impedance spectrum (BIS) measurement plates with three different electrode distances (1mm, 3mm, 5mm). Due to measurements using self-developed software, we got the result that plates with 3mm and 5mm distance setups are suitable for our future research studies. The validation measurement was carried out with series of solutions made of ion exchanged water and physiological saline solution (concentrations: 0%, 25%, 50%, 75%, and 100%) Due to successful plate development, it became possible to continuously measure the process of compaction and recovery of the dark neurons, and find out the details of this phenomenon.