MOESM4 of New insights into the heterogeneity of Th17 subsets contributing to HIV-1 persistence during antiretroviral therapy

Additional file 4. Superior Th17-lineage commitment of CM versus EM Th17 and Th1Th17-subsets. FACS-sorted Th17, Th1Th17 and Th1 subsets with CM (CD45RA−CCR7+) and EM (CD45RA−CCR7−) phenotype were cultured for 14 days under Th17-polarizing conditions, as in Fig. 5. Shown are the statistical analyses of the intracellular expression of IL-17A, IL-17F, IL-22, IFN-γ and TNF-α by distinct Th17-polarized CM (black bars) versus EM (white bars) Th17, Th1Th17 and Th1 subsets. Results (mean ± SEM) were generated with matched samples from n = 3 different subjects. Paired t-test p-values are indicated in the figures (CM versus EM).