HPLC Determination of a Metoclopramide and Ondansetron Mixture in 0.9% Sodium Chloride Injection

A high performance liquid chromatography procedure has been developed for the assay of a metoclopramide and ondansetron mixture in 0.9% sodium chloride injection. The separation and quantitation are achieved on a base deactivated octylsilane column at ambient temperature using a mobile phase of 77:23 v/v 0.01 M phosphate buffer, pH 4-acetonitrile at a flow rate of 1.0 mL/min with detection of analytes at 273 nm. The separation is achieved within 15–20 min with sensitivity in the ng/mL range for each analyte. The method showed linearity for metoclopramide and ondansetron in the 12.5–50 and 5–20 μ/mL ranges, respectively. Accuracy and precision were in the 1–2% and 0.3–1.3% ranges, respectively, for both drugs. The limits of detection for metoclopramide and ondansetron were 49 and 20 ng/mL, respectively, based on a signal to noise ratio of 3 and a 20 μL injection.