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Can co-culturing of two white-rot fungi increase lignin degradation and the production of lignin-degrading enzymes?
- Source :
- International Biodeterioration & Biodegradation. 59:32-39
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- The aim of this work was to investigate the poorly understood effects of co-culturing of two white rot fungi on the production of lignin-degrading enzyme activities. Four species, Ceriporiopsis subvermispora, Physisporinus rivulosus, Phanerochaete chrysosporium and Pleurotus ostreatus were cultured in pairs to study the degradation of aspen wood and the production of lignin-degrading enzymes. Potential of co-culturing for biopulping was evaluated. Chemical analysis of decayed aspen wood blocks showed that co-culturing of C. subvermispora with P. ostreatus could significantly stimulate wood decay, when compared to monocultures. Based on the fungi tested here, however, this effect is species-specific. Other combinations of fungi were slightly stimulating or not stimulatory. The pattern of lignin degradation was altered towards the acid insoluble part of lignin especially in co-cultures where P. ostreatus was included as a partner. The use of agar plates containing the polymeric dye Poly R-478 showed elevated dye decolourization at the confrontation zone between mycelia. Laccase was significantly stimulated only in the co-culture of P. ostreatus with C. subvermispora. Manganese peroxidase activity was stimulated in co-cultures of P. ostreatus with C. subvermispora or with P. rivulosus. Immunoblotting indicated changes in lignin-degrading enzymes and/or their isoform composition in response to co-culturing. This is the first report on the effects of co-culturing of potential biopulping fungi on wood degradation, and gives basic knowledge on fungal interactions during wood decay that can be utilized in practical applications.
- Subjects :
- Laccase
0303 health sciences
biology
030306 microbiology
fungi
technology, industry, and agriculture
Manganese peroxidase activity
15. Life on land
biology.organism_classification
complex mixtures
Microbiology
Biomaterials
Agar plate
03 medical and health sciences
chemistry.chemical_compound
chemistry
Botany
Phanerochaete
Lignin
Pleurotus ostreatus
Food science
Waste Management and Disposal
Mycelium
030304 developmental biology
Chrysosporium
Subjects
Details
- ISSN :
- 09648305
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- International Biodeterioration & Biodegradation
- Accession number :
- edsair.doi...........25ab84820e843bc8dbac9b9ddd13f75a