Effect of the ionic composition of a mobile phase on the chromatographic retention of profen enantiomers on a chiral adsorbent with grafted eremomycin antibiotic

The effect of ionic composition of mobile phase on retention and chromatographic separation of optical isomers of several derivatives of 2-arylpropanoic acid on a chiral stationary phase Diaspher-Chiralsel-E with a grafted eremomycin antibiotic was studied. As was shown, the mechanism of retention differs from that for hydrophobic reverse-phase adsorbents, as it involves a substantial ion-exchange component that determines the nonmonotonic dependence of the retention factor and the enantioselectivity on eluent pH. The effect of the concentration of buffer salt in the eluent on the chromatography of this class of compounds is discussed.