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miR-140-Modified Bone Marrow Mesenchymal Stem Cells Enhance Chemotherapy Sensitization in Cervical Squamous Cell Carcinoma Cells via Targeting Microtubule Depolymerization Protein 1 (STMN1)
- Source :
- Journal of Biomaterials and Tissue Engineering. 12:232-238
- Publication Year :
- 2022
- Publisher :
- American Scientific Publishers, 2022.
-
Abstract
- Effect of bone marrow mesenchymal stem cells (BMSCs) on the sensitivity of chemotherapy drugs and microRNAs (miRNAs) is still unclear. This study explored the role of miR-140 modified BMSCs in enhancing paclitaxel sensitivity of cervical squamous cell carcinoma (CSCC). Hela cells, BMSCs cells, and miR-140 modified BMSCs were transfected with miR-140 mimic, miR-140 inhibitor, and miR-140 NC, respectively. After transfection, they were co-cultured with Hela cells and paclitaxel to set up miR-140 mimic group, miR-140 inhibitor group, and miR-140 NC group (without paclitaxel treatment) followed by analysis of cell proliferation, apoptosis, ROS generation, expression of miR-140, STMN1, STAT3, p-STAT3, and survivin mRNA and protein. miR-140 inhibitor group showed lowest cell proliferation number and expressions of miR-140, STMN1, STAT3, p-STAT3, and survivin mRNA and protein with highest number of apoptotic cells, which were all reversed in miR-140 mimic group. There was a positive correlation between STMN1 level and miR-140 expression (r = 0.449, P = 0.108). BMSCs modified with miR-140 inhibitor can target STMN1, enhance the sensitivity of chemotherapy drugs, and exert an inhibitory effect on CSCC cell proliferation, suggesting that STMN1 might be a therapy target for treating CSCC.
- Subjects :
- Chemotherapy
Cervical Squamous Cell Carcinoma
business.industry
medicine.medical_treatment
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
Bone marrow mesenchymal stem cells
medicine.anatomical_structure
Microtubule Depolymerization
medicine
Cancer research
business
Sensitization
Biotechnology
Subjects
Details
- ISSN :
- 21579083
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Biomaterials and Tissue Engineering
- Accession number :
- edsair.doi...........24a7b619d46ebb875f9ab1aafc383318