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Turn-on detection of cysteine by a donor-acceptor type quinoline fluorophore: Exploring the sensing strategy and performance in bioimaging
- Source :
- Dyes and Pigments. 193:109556
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Tracking the biothiol cysteine (Cys) in living systems is a significant responsibility to balance the redox environment and oxidative stress. A quinoline-7-nitro-1,2,3-benzoxadiazole (Q-NBD) fluorophore has been synthesized and characterized towards examination of Cys. The probe forms a quinoline-substituted phenol (Q-Ph-OH) after thiolysis of the NBD ether bond, leading to an increase of fluorescence at green channel. The turn-on sensing mechanism originates from the change in intramolecular charge transfer (ICT-OFF) along with an aggregation-induced emission (AIE) as suggested by spectroscopy measurements in solutions, time-dependent density-functional theory (TD-DFT) calculations and 1H NMR titration examination. Importantly, Q-NBD exhibited great sensitivity with a low limit of detection value of 89.5 nM and remarkable selectivity in various biothiols towards Cys. The sensor probe was successfully used for detecting both endogenous and exogenous Cys in PC3 living cells and spiked Cys in human urine samples.
- Subjects :
- Fluorophore
Chemistry
Process Chemistry and Technology
General Chemical Engineering
Quinoline
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
Photochemistry
01 natural sciences
Fluorescence
0104 chemical sciences
chemistry.chemical_compound
Thiolysis
Intramolecular force
Titration
0210 nano-technology
Selectivity
Cysteine
Subjects
Details
- ISSN :
- 01437208
- Volume :
- 193
- Database :
- OpenAIRE
- Journal :
- Dyes and Pigments
- Accession number :
- edsair.doi...........23f319f9b41e669b1a3a2fa70f06a175