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Oxidative Stress Effect on Testicular Germ Cells and Liver Cells in Mice Induced by Dibutyl Phthalate
- Source :
- Applied Mechanics and Materials. :241-244
- Publication Year :
- 2013
- Publisher :
- Trans Tech Publications, Ltd., 2013.
-
Abstract
- Dibutyl phthalate (DBP) as a commonly used plasticizer can be detected in many environmental medium. In this study oxidative damage effect on testicular germ cells and liver cells in mice induced by DBP were investigated via vitro test. Testicular germ cells and liver cells of mice were incubated with medium containing DBP in different concentrations (0μmol/L,10μmol/ L,50μmol/L,100μmol/L), after one hour thiobarbituric acid assay and xanthine oxidase assay were used to measure the levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) respectively.The results showed that the levels of MDA in testicular germ cells and liver cells increased gradually with the increase of DBP concentrations, while compared with the control group, the activities of SOD declined significantly at dosage of 50μmol/L and 100μmol/L. These results suggested that DBP could cause lipid peroxidation in testicular germ cells and liver cells of mice in vitro. Oxidative damage may be one of its toxic mechanisms. It would provide an experimental foundation for security application of DBP.
- Subjects :
- endocrine system
biology
Dibutyl phthalate
Thiobarbituric acid
General Medicine
medicine.disease_cause
Malondialdehyde
In vitro
Andrology
Superoxide dismutase
Lipid peroxidation
chemistry.chemical_compound
chemistry
medicine
biology.protein
Xanthine oxidase
Oxidative stress
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 16627482
- Database :
- OpenAIRE
- Journal :
- Applied Mechanics and Materials
- Accession number :
- edsair.doi...........23d7b47b9be142d14391d98801a8ab9c