Identification and Quantification of Heterologous Compounds Parthenin and Organic Acids inParthenium HysterophorusL. Using HPLC-PDA-MS-MS

Parthenium hysterophorus L., is an obnoxious weed known for its environmental health hazards and medicinal uses. These characteristics are due to presence of sesquiterpene lactones and organic acids; therefore a rapid and sensitive analytical procedure using HPLC-PDA-MS-MS was developed and optimized for separation, identification, and quantification of parthenin and six organic acids. Separation and characterization of compounds was achieved on a RP-C18 column with 1% acetic acid in water (A) and acetonitrile (B) as a mobile phase at a flow rate of 0.6 mL min−1 and by matching their UV and mass spectra with reference compounds. Six organic acids (ferulic acid, 0.1 mg g−1 to coumaric acid, 13.6 mg g−1) and parthenin (27.4 mg g−1) were characterized within 26 minutes of chromatographic separation in plant extract. The calibration curves are linear with correlation coefficients from 0.985 to 0.998, limit of detection and quantification ranged between 1.0 µg mL−1 (anisic acid) to 2.2 µg mL−1 (parthenin) and ...