The use of stimulated primary spleen cell cultures in evaluating cell cycle response to toxicant insult

Isolated rodent spleen cells, cultured for 48 hours with the mitogen Concanavalin A (Con A), consistently provide a cycling population with which to test the cell cycle effects (both temporal and quantitative) of various toxicants. Any modification in the cell cycle is then analyzed by flow cytometric techniques and computer-aided statistical comparisons of the DNA histogram of the cell populations.