Verification of a quantitative method: complete blood count by flow cytometry, the HematoFlowTM system (Beckman Coulter)

The HematoFlow™ system is used in the hematology laboratory of the University Hospital of Bordeaux since July, 2011. The HematoFlow™ solution is the combination of a sample preparator (FP1000) and a 5 color flow cytometer (FC500) linked by a middleware (Remisol™). This system is used in second line when flags are activated by the hematology instrument and/or if the sample comes from the OncoHematology Department. Improvements in hematology disease diagnosis and follow-up were possible using this system. The laboratory has now entered in an accreditation procedure and needs to check this method in compliance with the COFRAC requirements.