Immunoassays for Leptin and Leptin Receptors

Availability of methods for accurate and reproducible quantification of lepth in various biological fluids in humans and in animals has greatly facilitated research leading to the understanding of its role in various physiological and pathophysiological conditions. Immunoassay techniques provide a simple and robust tool for measurement of biomolecules. In this chapter, I describe the principles behind various types of immunoassay methods, features of several commercially available leptin and leptin receptor immunoassays, and factors that influence leptin measurement using immunoassays.