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Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed
- Source :
- Food Analytical Methods. 9:2434-2443
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- To monitor the illegal use of florfenicol (FF) and thiamphenicol (TAP) in edible animal tissue and feed, a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed with simple sample preparation and cleanup. The obtained monoclonal antibody (5F4) that has isotype IgG1 showed an IC50 value of 0.21 μg L−1 for FF and 0.35 μg L−1 for TAP, respectively. It did not exhibit measurable cross-reactivity with other antibiotics. The limits of detection (LODs) for FF and TAP in a muscle matrix ranged from 0.07 to 0.14 μg kg−1 and in a feed matrix ranged from 2.9 to 5.2 μg kg−1. The recoveries were 72.8 to 113.4 % with a coefficient of variation of less than 15 %. Good correlation between the ELISA and HPLC-MS/MS results in the tissues tested demonstrated the reliability of our ic-ELISA. This ELISA is a useful tool for screening FF and TAP in edible animal tissue and feed.
- Subjects :
- Florfenicol
Detection limit
chemistry.chemical_classification
Chromatography
010405 organic chemistry
medicine.drug_class
Coefficient of variation
010401 analytical chemistry
Biology
Monoclonal antibody
Thiamphenicol
01 natural sciences
Applied Microbiology and Biotechnology
Isotype
0104 chemical sciences
Analytical Chemistry
Matrix (chemical analysis)
chemistry.chemical_compound
Enzyme
chemistry
medicine
Safety, Risk, Reliability and Quality
Safety Research
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi...........21eb116aff4ac3ba124dd1ab05540aec