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An add-on adaptive optical module for laser scanning microscopy (Conference Presentation)

Authors :
Yajie Liang
Na Ji
Daniel E. Milkie
Thomas G. Bifano
Ichun Anderson Chen
Wenzhi Sun
Source :
Adaptive Optics and Wavefront Control for Biological Systems IV.
Publication Year :
2018
Publisher :
SPIE, 2018.

Abstract

Wavefront-shaping devices incorporated into optical microscopy systems are capable of correcting sample-induced aberrations and recovering diffraction-limited imaging performance. The widespread dissemination and application of adaptive optical techniques, however, requires easy integration of adaptive optical modules, both in terms of hardware and software, into existing microscopes. We built an adaptive optical module with reduced complexity and simplified integration by utilizing a novel segmented deformable mirror and a standalone control software program. We demonstrated its ability to improve image brightness and resolution at depth in the mouse, zebrafish, and fly brains in vivo.

Details

Database :
OpenAIRE
Journal :
Adaptive Optics and Wavefront Control for Biological Systems IV
Accession number :
edsair.doi...........21d2551dd408e434b82089a12953109f
Full Text :
https://doi.org/10.1117/12.2292944