Simultaneous Determination of (2R,3R)-Dihydromyricetin and Myricetin of Mongolia Medicine Sendeng-4 in RAT Plasma by Liquid Chromatography–Mass Spectrometry

A liquid chromatography−mass spectrometry method was developed for the simultaneous quantitation of (2R,3R)-Dihydromyricetin and Myricetin in rat plasma samples to facilitate analysis of samples collected after administration of SenDeng-4 medicine. The plasma was subjected to protein precipitation with HCl (containing 10% ascorbic acid) and analyzed using electrospray ionization mass spectrometry in selected ion monitoring mode with Paeoniflorin as an internal standard. Good linearity for the two compounds was observed. The intra- and inter-day precision of analysis was