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Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus)
- Source :
- Environmental Toxicology. 32:979-988
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- The p53 pathways play an important role in carcinogenesis. In mammals, p53 and p53 target genes have been extensively studied, but little is known about their functions and regulation in fish. In this study, the cDNA fragments of p53 network genes, including p53, p21, mdm2, gadd45α, gadd45β, igfbp-3, and bax, were cloned from Chinese rare minnow (Gobiocypris rarus). These genes displayed high amino acid sequence identities with their zebrafish orthologs. The mRNA levels of p53 network genes and pathological changes in the liver were determined after adult rare minnow were exposed to 0.4, 2, and 10 µg/L of benzo[a]pyrene (BaP) for 28 days. The results showed that p53, p21, mdm2, gadd45α, and bax mRNA expressions in the livers from males and females were significantly upregulated compared with those of the controls (p
- Subjects :
- 0301 basic medicine
DNA repair
Health, Toxicology and Mutagenesis
010501 environmental sciences
Management, Monitoring, Policy and Law
Toxicology
medicine.disease_cause
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
biology.animal
Gene expression
medicine
Gene
Zebrafish
0105 earth and related environmental sciences
Genetics
biology
General Medicine
Minnow
biology.organism_classification
Molecular biology
030104 developmental biology
Benzo(a)pyrene
chemistry
Gobiocypris rarus
Carcinogenesis
Subjects
Details
- ISSN :
- 15204081
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Environmental Toxicology
- Accession number :
- edsair.doi...........20867289b5ff641355b1b67d3a4c4472