Preliminary results in improving essential fatty acids enrichment of rotifer cultured in high density

High density cultured marine rotifer (Brachionus rotundiformis) fed on freshwater Chlorella was cultured secondarily with an emulsion of ethyl esters (65% DHA, 15% EPA) for EFA-enrichment, with or without freshwater Chlorella. Culture tanks (30 1, 20 1 working volume) set in a water bath (24 °C) were continuously supplied with air (2.501 m−1) or high-purity oxygen gas (1.25 1 nn−1). Samples for fatty acid composition analysis were collected at 0, 6, 12, and 24 hours after the inoculation of rotifer, followed by the monitoring of rotifer density, DO, NH4-N and pH.