High throughput screening for identification of RANTES chemokine expression inhibitors

Publisher Summary This chapter discusses high throughput screening (HTS) for identification of RANTES chemokine. HTS is a process by which a large number of molecules can be tested for their effect on a biochemical or biological reaction. This process can be automated and is easily adapted to a 96-well microtiter format. Compounds for screening may come from existing chemical libraries, from combinatorial chemistry, or from natural sources. Using this screening protocol a potential lead compound that inhibits RANTES expression in the astrocytoma cell line CH235 have identified. In assay and experimental design the chapter utilizes a whole cell based assay with RANTES protein levels secreted into the medium determined by ELISA. Such methods do not allow for identification of mechanism of action for these compounds, which has yet to be determined.