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Adipose-derived aldehyde dehydrogenase-expressing cells promote dermal regenerative potential with collagen-glycosaminoglycan scaffold
- Source :
- Wound Repair and Regeneration. 25:109-119
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH-expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with collagen-glycosaminoglycan (c-GAG) scaffolds. ALDH-positive cells were isolated by a fluorescence-activated cell sorting (FACS) technique from Lewis rat's Stromal-Vascular-Fraction (SVF) and transplanted with c-GAG scaffolds in a rat full-thickness skin wound model. At seven days after surgery, the microscopic appearance of c-GAG scaffolds seeded with ALDH-positive was compared with those of uncultured-SVF, and cultured- adipose-derived stromal cells (ASCs). The thickness of cellular ingrowth in the ASC group (630 ± 180 μm) was significantly thicker than that in the control (390 ± 120 μm) or SVF (380 ± 140 μm) groups, but non-significantly thicker than that in the ALDH-positive group (570 ± 220 μm). The thickness of regenerated collagen layer was significantly thicker in the ALDH-positive group (160 ± 110 μm) than in the ASCs (81 ± 41 μm), the control (65 ± 24 μm), or SVF (64 ± 34 μm) groups. Immunofluorescent staining with CD31 proved that transplanted ALDH-positive cells differentiated into vascular endothelial cells in c-GAG scaffolds. Combined transplantation with c-GAG scaffolds and adipose-derived ALDH-positive cells promoted dermal regeneration, giving a possibility that ALDH-positive cells would greatly shorten the waiting period before secondary autologous skin grafting was possible. This article is protected by copyright. All rights reserved.
- Subjects :
- 0301 basic medicine
Stromal cell
Chemistry
Regeneration (biology)
medicine.medical_treatment
Adipose tissue
Dermatology
Anatomy
Molecular biology
Endothelial stem cell
Transplantation
Glycosaminoglycan
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
030220 oncology & carcinogenesis
medicine
Skin grafting
Surgery
Stem cell
Subjects
Details
- ISSN :
- 10671927
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Wound Repair and Regeneration
- Accession number :
- edsair.doi...........1cdd85e078aa38cfe642aea160470042