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Attenuated phagocytosis of secondary necrotic neutrophils by macrophages in aged and SMP30 knockout mice
- Source :
- Geriatrics & Gerontology International. 16:135-142
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Aim Secondary necrotic cells generated in vivo induce inflammatory responses; for example, the production of macrophage inflammatory protein-2 (MIP-2) and subsequent infiltration of neutrophils. The aim of the present study was to elucidate the effect of aging on the phagocytosis of secondary necrotic cells and the inflammatory responses by using either wild-type (WT) young mice, WT aged mice or senescence-accelerated mice (SMP30−/− mice). Methods The phagocytosis of secondary necrotic neutrophils with resident macrophage from either WT young mice, WT aged mice or SMP30−/− mice was examined by coculturing macrophages with secondary necrotic neutrophils in vitro. To investigate the inflammatory response induced by secondary necrotic cells, time-dependent infiltration of neutrophils and production of MIP-2 were determined in the peritoneal cavity on the injection of secondary necrotic cells. Results The phagocytosis of secondary necrotic cells by macrophages from WT aged and SMP30−/− mice was significantly reduced as compared with that by macrophages from WT young mice. On peritoneal injection of secondary necrotic cells, the peak time of neutrophil infiltration was earlier in SMP30−/− mice than in WT young mice. The number of neutrophils in SMP30−/− mice at the peak time was also greater than that in WT young mice. Conclusions Our findings showed that the phagocytosis of secondary necrotic cells was attenuated in aged mice and SMP30−/− mice, and that the MIP-2 production was enhanced and subsequently neutrophil infiltration was exaggerated on peritoneal injection of secondary necrotic cells into those mice. Geriatr Gerontol Int 2015; ●●: ●●–●●.
- Subjects :
- 0301 basic medicine
Chemokine
Pathology
medicine.medical_specialty
biology
business.industry
Phagocytosis
Inflammatory response
Inflammation
medicine.disease
03 medical and health sciences
Peritoneal cavity
030104 developmental biology
medicine.anatomical_structure
Apoptosis
Knockout mouse
medicine
biology.protein
medicine.symptom
business
Infiltration (medical)
Subjects
Details
- ISSN :
- 14441586
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Geriatrics & Gerontology International
- Accession number :
- edsair.doi...........1c5156d89bf44a55347bb2ffe6481311