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Long-Term Storage Effects on Stability of Aβ1–40, Aβ1–42, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays
- Source :
- Dementia and Geriatric Cognitive Disorders Extra. 9:77-86
- Publication Year :
- 2019
- Publisher :
- S. Karger AG, 2019.
-
Abstract
- Background: The stability of Alzheimer’s disease (AD) biomarkers in plasma, measured by immunomagnetic reduction (IMR) after long-term storage at –80°C, has not been established before. Method: Ninety-nine human plasma samples from 53 normal controls (NCs), 5 patients with amnestic mild cognitive impairment (aMCI), and 41 AD patients were collected. Each plasma sample was aliquoted and stored as single-use aliquots at –80°C. The baseline measurements for Aβ1–40, Aβ1–42, and total Tau protein (T-Tau) concentrations for each sample were done within 3 months of blood draw by IMR. They are referred to as baseline concentrations. A separate aliquot from each sample was assayed with IMR to assess the stability of the measured analytes during storage at –80°C between 1.1 and 5.4 years. This is referred to as a repeated result. Results: IMR shows that plasma levels of Aβ1–40 and Aβ1–42 exhibit stability over 5-year storage at –80°C and that plasma levels of T-Tau are less stable (approximately 1.5 years). Conclusion: Although the measured concentrations of T-Tau in human plasma may alter during storage, the diagnostic utility of the results are only slightly affected when the product of Aβ1–42 and T-Tau concentrations are used. The results show that the overall agreement between baseline and repeated measurements in the ability of discriminating NCs from aMCI/AD patients is higher than 80%.
- Subjects :
- Analyte
030214 geriatrics
Plasma samples
Chemistry
Cognitive Neuroscience
Total tau
Plasma levels
Andrology
03 medical and health sciences
Psychiatry and Mental health
Blood draw
0302 clinical medicine
Human plasma
mental disorders
Total Tau Protein
Cognitive impairment
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 16645464
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Dementia and Geriatric Cognitive Disorders Extra
- Accession number :
- edsair.doi...........19d3310ad8a7bf0abeccc7c914ec65ae