Abstract 3912: Comprehensive Characterization of SOX4 Protein Complexes in Prostate Cancer Cells

Title: Comprehensive Characterization of SOX4 Protein Complexes in Prostate Cancer Cells. Authors: Yu Heng Lai, Junmin Peng, Jessica Cheng, and Carlos S. Moreno Abstract: SOX4 is a developmental transcription factor in vertebrates that is required for differentiation and proliferation in multiple tissues. SOX4 is overexpressed in many human malignancies, but the precise role of SOX4 in cancer progression is still not well understood. Although it is known that SOX4 directly interacts with p53 and β-catenin, many of SOX4's protein binding partners remain unknown. Thus, the identification of additional SOX4 binding partners is essential for elucidating the mechanism of SOX4-mediated effects in cancer progression. We have adapted a one-step affinity purification method that allows rapid purification of via intracellular biotinylation of the amino-terminus of SOX4. We have transiently expressed SOX4 protein in LNCaP prostate cancer cells and purified SOX4 complexes with streptavidin-linked paramagnetic beads in order to perform large scale of proteomics analysis. We have identified several proteins that interact with SOX4 in vivo by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Details of these SOX4 protein-protein binding partners will be discussed. These proteomic data provide insights into the role of SOX4 in key pathways in cell proliferation, development, and cancer progression. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3912.