Back to Search
Start Over
A novel ATP-binding cassette transporter from Leishmania is involved in transport of phosphatidylcholine analogues and resistance to alkyl-phospholipids
- Source :
- Molecular Microbiology. 64:1141-1153
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- ATP-binding cassette (ABC) transporters represent an important family of membrane proteins involved in drug resistance and other biological activities. The present work reports the characterization of the first ABC subfamily G (ABCG)-like transporter, LiABCG4, in the protozoan parasite Leishmania. LiABCG4 localized mainly to the parasite plasma membrane. Overexpression of this half-transporter reduced the accumulation of phosphatidylcholine analogues and conferred resistance to alkyl-phospholipids. Likewise, when expressed in Saccharomyces cerevisiae, the protein localized to the yeast plasma membrane and conferred resistance to alkyl-phospholipids. Post-Golgi secretory vesicles isolated from a LiABCG4-overexpressing yeast mutant contained the leishmanial ABC transporter and exhibited ATP-dependent, vanadate-sensitive transport of phosphatidylcholine analogues from the cytosolic to the lumenal leaflet of the vesicle membrane. Cross-linking showed dimerization of LiABCG4. These results suggest that LiABCG4 is involved in the active transport of phosphatidylcholine and resistance to alkyl-phospholipids in Leishmania.
- Subjects :
- biology
Saccharomyces cerevisiae
Phospholipid
ATP-binding cassette transporter
Transporter
biology.organism_classification
Microbiology
Secretory Vesicle
Cytosol
chemistry.chemical_compound
chemistry
Biochemistry
Membrane protein
Phosphatidylcholine
lipids (amino acids, peptides, and proteins)
Molecular Biology
Subjects
Details
- ISSN :
- 0950382X
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi...........198dfd1d66a336ea19d1f8e3fa711ab3