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Application of viral vectors to the study of neural connectivities and neural circuits in the marmoset brain
- Source :
- Developmental Neurobiology. 77:354-372
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- It is important to study the neural connectivities and functions in primates. For this purpose, it is critical to be able to transfer genes to certain neurons in the primate brain so that we can image the neuronal signals and analyze the function of the transferred gene. Toward this end, our team has been developing gene transfer systems using viral vectors. In this review, we summarize our current achievements as follows. 1) We compared the features of gene transfer using five different AAV serotypes in combination with three different promoters, namely, CMV, mouse CaMKII (CaMKII), and human synapsin 1 (hSyn1), in the marmoset cortex with those in the mouse and macaque cortices. 2) We used target-specific double-infection techniques in combination with TET-ON and TET-OFF using lentiviral retrograde vectors for enhanced visualization of neural connections. 3) We used an AAV-mediated gene transfer method to study the transcriptional control for amplifying fluorescent signals using the TET/TRE system in the primate neocortex. We also established systems for shRNA mediated gene targeting in a neocortical region where a gene is significantly expressed and for expressing the gene using the CMV promoter for an unexpressed neocortical area in the primate cortex using AAV vectors to understand the regulation of downstream genes. Our findings have demonstrated the feasibility of using viral vector mediated gene transfer systems for the study of primate cortical circuits using the marmoset as an animal model. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 354-372, 2017.
- Subjects :
- 0301 basic medicine
Neocortex
biology
Gene targeting
Marmoset
Macaque
Viral vector
Small hairpin RNA
03 medical and health sciences
Cellular and Molecular Neuroscience
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Developmental Neuroscience
biology.animal
medicine
Transcriptional regulation
Biological neural network
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 19328451
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Developmental Neurobiology
- Accession number :
- edsair.doi...........1980ba0a21ea82738af954b8efe5b45f