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pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy for sensitive detection and live cell imaging of multiple microRNAs
- Source :
- Sensors and Actuators B: Chemical. 293:93-99
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Here, a pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy was proposed for sensitive detection and live cell imaging of multiple microRNAs. The nanoprobe including ZnO nanoparticles (ZnO NPs) core as carrier and cofactor provider and polydopamine shell for adsorption the functional hairpin DNAs was designed. When nanoprobe entered the cell through endocytosis, the acidic environment of the cell could decompose the ZnO NPs core to release the functional hairpin DNAs and Zn2+. The Zn2+ could act as cofactor for the DNAzyme cleavage amplification reaction, avoiding additional cell delivery processes. The recognition hairpin DNAs (H1 and H3) recognized microRNA and exposed the DNAzyme. The DNAzyme cleaved cyclically the reporter hairpin DNAs (H2 and H4), producing enhanced fluorescent signal for miR-21 and miR-373 detection with the detection limit of 54 pM and 38 pM, respectively. Expression levels of miR-21 in Hela, HepG-2 and L02 cells were differentiated. Furthermore, simultaneous imaging of miR-21 and miR-373 in same living cells was achieved. These results indicated this strategy could have a potential application in microRNAs assays for the accurate diagnosis and therapy of cancer.
- Subjects :
- Cell
Deoxyribozyme
Nanoprobe
02 engineering and technology
010402 general chemistry
Cleavage (embryo)
Endocytosis
01 natural sciences
HeLa
Live cell imaging
Materials Chemistry
medicine
Electrical and Electronic Engineering
Instrumentation
biology
Chemistry
Metals and Alloys
021001 nanoscience & nanotechnology
Condensed Matter Physics
biology.organism_classification
Fluorescence
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
medicine.anatomical_structure
Biophysics
0210 nano-technology
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 293
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi...........18332102b176bd1671abb9ed7a21f967