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3H-tetracycline as a proxy for 41Ca for measuring dietary perturbations of bone resorption
- Source :
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 259:790-795
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Our group is interested in evaluating early effects of dietary interventions on bone loss. Postmenopausal women lose bone following reduction in estrogen which leads to increased risk of fracture. Traditional means of monitoring bone loss and effectiveness of treatments include changes in bone density, which takes 6 months to years to observe effects, and changes in biochemical markers of bone turnover, which are highly variable and lack specificity. Prelabeling bone with 41Ca and measuring urinary 41Ca excretion with accelerator mass spectrometry provides a sensitive, specific, and rapid approach to evaluating effectiveness of treatment. To better understand 41Ca technology as a tool for measuring effective treatments on reducing bone resorption, we perturbed bone resorption by manipulating dietary calcium in rats. We used 3H-tetracycline (3H-TC) as a proxy for 41Ca and found that a single dose is feasible to study bone resorption. Suppression of bone resorption, as measured by urinary 3H-TC, by dietary calcium was observed in rats stabilized after ovariectomy, but not in recently ovariectomized rats.
- Subjects :
- Nuclear and High Energy Physics
medicine.medical_specialty
Bone density
business.industry
medicine.drug_class
chemistry.chemical_element
Calcium
Bone resorption
Bone remodeling
Excretion
Endocrinology
chemistry
Estrogen
Internal medicine
medicine
Ovariectomized rat
business
Densitometry
Instrumentation
Subjects
Details
- ISSN :
- 0168583X
- Volume :
- 259
- Database :
- OpenAIRE
- Journal :
- Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
- Accession number :
- edsair.doi...........180169f28b567b8bc5b65225c84fd9c7