Geo-magnetic cues influence on naïve learning of Australian red honey ant

Introduction: The ability of ants to navigate and locate resources is a remarkable adaptation that has been widely studied. While many factors influence ant navigation, recent research has shown that geo-magnetic cues play a crucial role in guiding their orientation and homing behaviors. However, the specific influence of geo-magnetic cues on naïve learning, particularly in the context of Australian red honey ants (Melophorus spp.), remains poorly understood. This pre-registration project aims to investigate the role of geo-magnetic cues in naïve learning and spatial memory formation of Australian red honey ants. Hypotheses: Naïve Australian red honey ants will exhibit an innate preference for specific geo-magnetic field characteristics. Exposure to altered or disrupted geo-magnetic cues will impair the naïve learning and spatial memory abilities of Australian red honey ants. Methods: Experimental Design: Two experimental groups will be created: the control group and the disrupted geo-magnetic cues group. The control group will experience natural geo-magnetic cues, while the disrupted geo-magnetic cues group will be exposed to manipulated magnetic fields. The magnetic field manipulation will be achieved using electromagnets or custom-designed magnetic field generators. Data Analysis: the accuracy of navigation will be analyzed using appropriate statistical methods. Group comparisons will be conducted to determine the influence of disrupted geo-magnetic cues on naïve learning and spatial memory abilities. Conclusion: This pre-registration project aims to investigate the influence of geo-magnetic cues on naïve learning and spatial memory formation in Australian red honey ants. By manipulating the geo-magnetic cues and conducting controlled experiments, we seek to enhance our understanding of the sensory mechanisms underlying ant navigation and provide valuable insights into the ecological adaptation of these fascinating insects. The results of this study can contribute to our knowledge of magnetoreception in ants and have broader implications for the understanding of animal navigation and orientation behaviors.