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Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post-larval Penaeus vannamei (Bonne)

Authors :
A B Montero
Brian Austin
Source :
Journal of Fish Diseases. 22:377-386
Publication Year :
1999
Publisher :
Wiley, 1999.

Abstract

Vibrio harveyi recovered from diseased post-larval Penaeus vannamei produced a thermostable exotoxin, which was lethal to Dublin Bay prawns, Nephrops norvegicus L., when injected intramuscularly. The extracellular products (ECPs) concentrated from tryptone soya broth supplemented with 1% (w/v) sodium chloride or from cellophane overlays on marine 2216E agar with incubation at 15, 22 and 27 °C were toxic, with the lethal dose 50% of the crude ECPs estimated to be 4.4 μgprotein prawn−1. Proteolytic, haemolytic and cytotoxic activities were detected, although the occurrence and quantity of these activities were influenced by cultural conditions. The ECPs which had been heated (100 °C for 10 min) or digested with protease K produced the same pathology as crude, untreated ECPs. Western blotting demonstrated that all the ECP preparations contained low molecular weight lipopolysaccharides, which may constitute the lethal toxin of V. harveyi.

Details

ISSN :
13652761 and 01407775
Volume :
22
Database :
OpenAIRE
Journal :
Journal of Fish Diseases
Accession number :
edsair.doi...........1676ab6ea921771654d038af66e3da20
Full Text :
https://doi.org/10.1046/j.1365-2761.1999.00189.x