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Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post-larval Penaeus vannamei (Bonne)
- Source :
- Journal of Fish Diseases. 22:377-386
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- Vibrio harveyi recovered from diseased post-larval Penaeus vannamei produced a thermostable exotoxin, which was lethal to Dublin Bay prawns, Nephrops norvegicus L., when injected intramuscularly. The extracellular products (ECPs) concentrated from tryptone soya broth supplemented with 1% (w/v) sodium chloride or from cellophane overlays on marine 2216E agar with incubation at 15, 22 and 27 °C were toxic, with the lethal dose 50% of the crude ECPs estimated to be 4.4 μgprotein prawn−1. Proteolytic, haemolytic and cytotoxic activities were detected, although the occurrence and quantity of these activities were influenced by cultural conditions. The ECPs which had been heated (100 °C for 10 min) or digested with protease K produced the same pathology as crude, untreated ECPs. Western blotting demonstrated that all the ECP preparations contained low molecular weight lipopolysaccharides, which may constitute the lethal toxin of V. harveyi.
- Subjects :
- food.ingredient
biology
Vibrio harveyi
Veterinary (miscellaneous)
education
Aquatic Science
biology.organism_classification
medicine.disease_cause
Median lethal dose
Microbiology
chemistry.chemical_compound
fluids and secretions
food
chemistry
Tryptone
Extracellular
medicine
Agar
Penaeus
Incubation
Exotoxin
Subjects
Details
- ISSN :
- 13652761 and 01407775
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Journal of Fish Diseases
- Accession number :
- edsair.doi...........1676ab6ea921771654d038af66e3da20
- Full Text :
- https://doi.org/10.1046/j.1365-2761.1999.00189.x