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T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
- Source :
- Methods in Molecular Biology ISBN: 9781493926930
- Publication Year :
- 2015
- Publisher :
- Springer New York, 2015.
-
Abstract
- The ELISPOT is a powerful functional assay used to detect biological activity and immunological secretions from immune cells. In this chapter, we specifically discuss T cell ELISPOT methods for the detection of secreted cytokines. A detailed protocol is given enabling the detection of interferon gamma-secreting CD8(+) T cells and/or peripheral blood mononuclear cells following their isolation and polyclonal activation. Included is a brief discussion on choosing the activation method for your T cell ELISPOT assay, as well as additional instructions for the adaptation of this protocol for the study of memory and antigen-specific T cell responses.
Details
- ISBN :
- 978-1-4939-2693-0
- ISBNs :
- 9781493926930
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493926930
- Accession number :
- edsair.doi...........16633e34629dd7561bbd386069e9c662