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Induction of L-form Bacteria from Bacillus thuringiensis
- Source :
- Ceylon Journal of Science (Biological Sciences). 41:137-144
- Publication Year :
- 2013
- Publisher :
- Sri Lanka Journals Online (JOL), 2013.
-
Abstract
- Induction of L-form bacteria from Bacillus thuringiensis was investigated with the long-term view of their use as targeted biological control agents within plants. L-forms were successfully induced from B. thuringiensis subspecies israelens is 36-3 by preparing pour plates of exponential cultures in L-phase medium (LPM) containing μg ml -1 cephalosporin C. The induced L-forms were then subcultured and maintained in L-phase medium supplemented with inactivated mycoplasma screened 5% (v/v) horse serum (5HS) with a combination of 225 μg ml -1 cephalosporin C and 0.6 mg ml -1 penicillin G, using the push block method. Induced L-forms showed typical features with pleiomorphic cells that contained granular particles and small vacuoles but no parasporal bodies. These L-forms were capable of growing on solidified media but failed to grow in liquid media. Ceylon Journal of Science ( Bio. Sci. ) 41 (2): 137-144, 2012 DOI: http://dx.doi.org/10.4038/cjsbs.v41i2.5383
Details
- ISSN :
- 00692379
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Ceylon Journal of Science (Biological Sciences)
- Accession number :
- edsair.doi...........1647ceb519302ec7924d77aa172ae6c9