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Towards a biofunctionalized vascular prosthesis: immune cell trapping via a growth factor receptor

Authors :
Miriam Wedel
Burkhard Schlosshauer
Fritz Grunert
John F. Thompson
Sven Liebler
Source :
Journal of Tissue Engineering and Regenerative Medicine. 11:2699-2709
Publication Year :
2016
Publisher :
Hindawi Limited, 2016.

Abstract

To improve the clinical performance of vascular prostheses, which is inacceptably low for implants with small diameters ( 10 000 hybridoma clones were generated. Screening with various deletion mutants of VEGFR-2, 49 highly-specific monoclonal antibodies (mAbs) covering all seven Ig domains of VEGFR-2 were selected. mAb 9H10 was characterized in detail. Once immobilized on synthetic surfaces, mAb 9H10 allowed, within min, nearly 100-fold enrichment of VEGFR-2-expressing cells under continuous flow conditions. Cell trapping was cell-type specific and essentially not affected by competing VEGFR-2-negative cells. To exclude that the antibody would adversely modify receptor responses, four different in vitro assays were employed. Cell proliferation, angiogenic tube formation, acetylated low-density lipoprotein uptake and VEGFR-2 phosphorylation remained unaffected, suggesting that the antibody did not interfere with the receptor functioning of human umbilical vascular endothelial cells. The molecular and cellular characteristics make the selected monoclonal antibody a very promising tool for the biofunctionalization of vascular implants. Copyright © 2016 John Wiley & Sons, Ltd.

Details

ISSN :
19326254
Volume :
11
Database :
OpenAIRE
Journal :
Journal of Tissue Engineering and Regenerative Medicine
Accession number :
edsair.doi...........15f4486577d873ec1acd77259d0c42d2
Full Text :
https://doi.org/10.1002/term.2165