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Towards a biofunctionalized vascular prosthesis: immune cell trapping via a growth factor receptor
- Source :
- Journal of Tissue Engineering and Regenerative Medicine. 11:2699-2709
- Publication Year :
- 2016
- Publisher :
- Hindawi Limited, 2016.
-
Abstract
- To improve the clinical performance of vascular prostheses, which is inacceptably low for implants with small diameters ( 10 000 hybridoma clones were generated. Screening with various deletion mutants of VEGFR-2, 49 highly-specific monoclonal antibodies (mAbs) covering all seven Ig domains of VEGFR-2 were selected. mAb 9H10 was characterized in detail. Once immobilized on synthetic surfaces, mAb 9H10 allowed, within min, nearly 100-fold enrichment of VEGFR-2-expressing cells under continuous flow conditions. Cell trapping was cell-type specific and essentially not affected by competing VEGFR-2-negative cells. To exclude that the antibody would adversely modify receptor responses, four different in vitro assays were employed. Cell proliferation, angiogenic tube formation, acetylated low-density lipoprotein uptake and VEGFR-2 phosphorylation remained unaffected, suggesting that the antibody did not interfere with the receptor functioning of human umbilical vascular endothelial cells. The molecular and cellular characteristics make the selected monoclonal antibody a very promising tool for the biofunctionalization of vascular implants. Copyright © 2016 John Wiley & Sons, Ltd.
- Subjects :
- 0301 basic medicine
Tube formation
Cell growth
medicine.drug_class
Biomedical Engineering
Medicine (miscellaneous)
030204 cardiovascular system & hematology
Biology
Monoclonal antibody
Molecular biology
Biomaterials
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Immune system
Growth factor receptor
medicine
biology.protein
Phosphorylation
Antibody
Receptor
Subjects
Details
- ISSN :
- 19326254
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Tissue Engineering and Regenerative Medicine
- Accession number :
- edsair.doi...........15f4486577d873ec1acd77259d0c42d2
- Full Text :
- https://doi.org/10.1002/term.2165