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Molecular Minimal Residual Disease Monitoring in Acute Myeloid Leukemia
- Source :
- The Journal of Molecular Diagnostics. 20:389-397
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The ability to sensitively monitor minimal residual disease (MRD) has played a key role in improving the management and outcomes for a number of leukemias, particularly acute promyelocytic leukemia and childhood acute lymphoblastic leukemia. By contrast, MRD monitoring in acute myeloid leukemia (AML) has been limited by variable assay methodologies and a relative paucity of patient-specific MRD markers. Inter- and intratumor genetic heterogeneity poses significant challenges for the identification of molecular markers suitable for MRD monitoring in AML, particularly for those cases without structural chromosomal rearrangements associated with fusion genes. Furthermore, the need to discriminate which mutations may be suitable for MRD monitoring creates additional complexity. The mainstay of current molecular MRD monitoring is real-time quantitative PCR, targeting fusion genes, mutations, and gene overexpression. New technologies, particularly next-generation sequencing approaches, offer new ways to overcome these limitations. Here, the authors review the techniques available for molecular MRD monitoring in AML and discuss their utility in clinical practice.
- Subjects :
- 0301 basic medicine
Oncology
Acute promyelocytic leukemia
medicine.medical_specialty
Myeloid
Genetic heterogeneity
business.industry
Myeloid leukemia
medicine.disease
Minimal residual disease
Pathology and Forensic Medicine
body regions
Fusion gene
03 medical and health sciences
Leukemia
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
hemic and lymphatic diseases
030220 oncology & carcinogenesis
Internal medicine
medicine
Molecular Medicine
business
Childhood Acute Lymphoblastic Leukemia
Subjects
Details
- ISSN :
- 15251578
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The Journal of Molecular Diagnostics
- Accession number :
- edsair.doi...........151b611269fee81e6ec2ab2dc8041697