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Biofilm formation by South African non-O157 Shiga toxigenic Escherichia coli on stainless steel coupons
- Source :
- Canadian Journal of Microbiology. 66:328-336
- Publication Year :
- 2020
- Publisher :
- Canadian Science Publishing, 2020.
-
Abstract
- This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on both the submerged surface and the air–liquid interface of coupons, whereas strains O116:H21, wzx-Onovel5:H19, O129:H21, and O26:H11 formed biofilm only at the air–liquid interface. Viable cell counts and scanning electron microscopy showed that biofilm formation increased (p < 0.05) over time. The biofilm-forming ability of non-O157 STEC was strongest (p < 0.05) at 22 °C after 48 h of incubation. The strongest biofilm former regardless of temperature was O129:H23. Generally, at 10 °C, weak to no biofilm was observed for isolates O154:H10, O116:H21, wzx-Onovel5:H19, O26:H11, and O129:H21 after 168 h. This study found that temperature affected the biofilm-forming ability of non-O157 STEC strains. Overall, our data indicate a high potential for biofilm formation by the isolates at 22 °C, suggesting that non-O157 STEC strains could colonize stainless steel within food-processing facilities. This could serve as a potential source of adulteration and promote the dissemination of these potential pathogens in food.
- Subjects :
- 0303 health sciences
030306 microbiology
Chemistry
Immunology
Biofilm
General Medicine
biochemical phenomena, metabolism, and nutrition
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
Non o157
03 medical and health sciences
fluids and secretions
Genetics
medicine
bacteria
Shiga-Toxigenic Escherichia coli
Molecular Biology
Escherichia coli
030304 developmental biology
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi...........14ff4b6e371ee6e6f9c1fcac42ebf3f8