Biological study of hypertrophy sorosis scleroteniosis and its molecular characterization based on LSU rRNA

During this work, the pathogen agent of hypertrophy sorosis scleroteniosis was cultivated on artificial culture, and based on the morphological characteristics, the causal agent was identified as Sclerotinia sclerotiorum. The amplification of large subunit ribosomal RNA (LSU rRNA) was done, furthermore LSU rRNA was sequenced. Result shows that the molecular weight was 612 bp, and compared with the known LSU rRNAs sequence of different S. sclerotiorum strains found from NCBI database. On the molecular level, the pathogen agent of hypertrophy sorosis scleroteniosis was S. sclerotiorum. Biological characteristics showed that S. sclerotiorum mycelia can produce oxalic acid during the growth process, but they did not show any sclerotia form in liquid culture. Key words: Apothecia, hyphae, hypertrophy sorosis scleroteniosis, Sclerotinia sclerotiorum.