Reproductive and fetal repercussions of treatment with an aqueous extract of flowers of Hibiscus rosa sinensis in diabetic pregnant rats

s / Placenta 36 (2015) 469e521 513 Methods: Diabetes was induced by streptozotocin (40 mg/kg) in virgin female Wistar rats. After diabetes induction, rats were mated. Hancornia speciosa leaf aqueous extract (600 mg/kg) was administered from day 0 to 20 of pregnancy. On day 21 of pregnancy, all rats were anesthetized and killed. The uteri were removed to obtain maternal-fetal data. Results: Treatment with aqueous extract of H. speciosa showed no hypoglycemic effect in non-diabetic and diabetic animals (p>0.05). However, the plant treatment decreased fetal weight (4.9±0.6 vs control-5.1±0.5) and increased the preimplantation loss rate (23.1% vs control-3.9%) and skeletal anomalies (70.0% vs control-50.0%) in non-diabetic dams. The diabetic group presented a decrease in the number of live fetuses, maternal weight gain, gravid uterus weight and fetal weight, and an increase in the preand post-implantation loss rate, placental weight and index, and visceral and skeletal anomalies. The plant treatment did not alter any parameter in the diabetic group compared to the non-treated diabetic group (p>0.05). Conclusion: The aqueous extract from Hancornia speciosa leaves did not modify the maternal hyperglycemia and fetal anomalies. Therefore, the plant altered the reproductive outcome and was teratogenic in non-diabetic rats, demonstrating the importance of care in the use of substances during pregnancy. PB.23. REPRODUCTIVE AND FETAL REPERCUSSIONS OF TREATMENT WITH AN AQUEOUS EXTRACT OF FLOWERS OF HIBISCUS ROSA SINENSIS IN DIABETIC PREGNANT RATS G.T. Volpato , L.A.F. Afiune , T. Leal-Silva , K.E. Campos , D.C. Damasceno . 1 Institute of Biological and Health Sciences, Federal University of Mato Grosso, Brazil; 2 Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, UNESP, Brazil Hibiscus rosa sinensis, commonly known as rose mallow, is widely used in Brazilian folk medicine for the diabetes treatment. Objective: To evaluate the effect of Hibiscus rosa sinensis aqueous extract treatment on the reproductive outcomes and fetal anomaly incidence in diabetic and non-diabetic rats. Methods: Diabetes was induced by Streptozotocin (40 mg/kg) in virgin female Wistar rats. After diabetes induction, the rats were mated. Oral administration of an aqueous extract of Hibiscus rosa sinensis flowers was given to non-diabetic and diabetic pregnant rats at increasing doses: 100 mg/kg from day 0 to 7 of pregnancy, 200 mg/kg from day 8 to 14 and 400 mg/kg from day 15 to 20. On day 21 of pregnancy, all rats were anesthetized and killed. The uteri were removed to obtain maternal-fetal data. Results: Treatment with aqueous extract of H. rosa sinensis showed no hypoglycemic effect and did not alter the rate of congenital anomalies in non-diabetic and diabetic animals (p>0.05). The diabetic group presented a decrease in the number of live fetuses (9.2±2.7 vs 12.0±1.6-control), maternal weight gain (69.3±21.9 vs 116.7±12.7-control), gravid uterus weight (52.4±24.8 vs 75.9±9.1-control) and fetal weight, and an increase in the pre(21.3% vs 4.0%-control) and post-implantation (18% vs 7.6%control) loss rate, placental weight and index, and visceral and skeletal anomalies. The plant treatment decreased the pre(6.3%) and post-implantation (12.8%) loss rate, improved the gravid uterus weight (69.0±20.8) and maternal weight gain (96.8±43.5). Conclusion: Although we could not confirm its antidiabetic effect, the intervention with the Hibiscus rosa sinensis aqueous extract treatment did not present teratogenic effects and contributed to the improvement in reproductive performance in the diabetic rats. PB.24. REPRODUCTIVE OUTCOME AND BIOCHEMICAL PROFILE OF TREATMENT WITH CROTON URUCURANA LATEX IN PREGNANT RATS G.T. Volpato , T.S. Soares , R.Q. Moares-Souza , N.C. Orleanne , K.E. Campos , D.C. Damasceno . 1 Institute of Biological and Health Sciences, Federal University of Mato Grosso, Brazil; 2 Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu Medical School, UNESP, Brazil Croton urucurana, commonly known as dragon blood, is widely known in Brazilian folk medicine to have an abortifacient effect. Objective: To evaluate the effect of Croton urucurana latex treatment in pregnant rats on maternal reproductive outcome and biochemical profile. Methods: Virgin female Wistar rats were used and mated. Pregnant rats were randomized into four groups (minimum n1⁄4 12 animals/group): G11⁄4 control, G2 to G41⁄4 treatedwith Croton urucurana latex at doses of 200, 400 and 800mg/kg, respectively. Rats were orally treated by gavage, with plant latex or vehicle during all the gestational period. On gestational day 21, the rats were anesthetized and the blood collected for serum biochemical measurements. The gravid uteri were removed to obtain maternal data. Results: Treatment with the highest dose of C. urucurana showed an increase in protein (5.5±1.5 g/dL), cholesterol (144.0±58.8 mg/dL), alanine aminotransferase (ALT 81.5±20.7 U/L) and aspartate aminotransferase (AST 400.6±90.9 U/L) compared to the other groups (p 0.05). The post-implantation loss increased in the vaccinated group (14.7%), compared to the control (5.0%) and adjuvant (4.4%) groups. The gravid uterus weight was different in the adjuvant group (97.5±17.6 g) compared to the control (71.7 ± 20.2 g) and vaccinated (82.8 ± 17.3 g) groups. Other reproductive outcome parameters presented no alterations. Conclusion: Immunization was confirmed by increased IgG immunoglobulin, which assures the vaccination process, but the protein presented abortive effect. Thus, this vaccine requires carefulness in its use during pregnancy period. PB.26. EXPRESSION OF IGG AND ITS FC GAMMA RECEPTOR IN SWINE PLACENTAL TISSUES IN DIFFERENT GESATIONAL PERIODS Ad.C. Garro , M.G. Garcia , M.A. Koncurat . Biologia General, Facultad de Ciencias Veterinarias, Argentina; Histologia 1 y 2, Facultad de Ciencias Veterinarias, Universidad Nacional de La Pampa, General Pico, La Pampa