Incorporation of Radioiodine in Thyroid Hormones for the Determination of T3/T4Ratio Using Paper Chromatography

A precise and accurate method is being developed for the determination of T3/T4 ratio. This method depends on using serum of different thyroid cases for the assesment of T3/T4 ratio. It has been found that the best temperature of storage is 0.0 °C to 4 °C before extracting the iodinated tyrosines and thyronines. Iodination with 125I was done according to our previous technique to render the separated thyronine radioactive. Scanning method helped for the determination of T3/T4 ratio. The results were confirmed by sensitivity and recovery test and statistically analyzed. 100 cases were investigated comprising hypo-, eu-, and hyperthyroid cases.