Post-Pneumonectomy Septal Retraction and Remodeling Are Structural Determinants of Lung Regeneration

METHODS: A lentivirus system was used to generate miR-372overexpressing A549 lung adenocarcinoma cells. Proliferation, migration and invasiveness of the cells, their ability to form colonies in culture and tumors in mice, and their gene expression profiles were compared against those of control cells. Targeting of specific genes by miR-372 was studied by quantifying the effect of microRNA overexpression on RNA and protein levels, and using a synthetic luciferase gene with a miR-372 target site.