Challenges and Future Trends in DNA Microarray Analysis

Since its introduction, the DNA microarray platform has experienced a tremendous growth and currently it is a powerful tool used in various biological applications. However, it has not been embraced in the molecular diagnostic market as much as it was anticipated in the early days. The challenge comes from the skepticism on the reproducibility of the microarray data and on the reliability of the biological interpretations inferred. In addition, the microarray technology is suffering from strong competition by other diagnostic techniques. On the other hand, integration of various steps of the microarray assay into a harmonized and miniaturized handheld device suitable for point-of-care (POC) has been a goal for the microarray community. In this respect, significant progress has been achieved in coupling the DNA microarrays with efficient liquid manipulation microsystems as well as in developing novel technologies of supporting subsystems that well suits future POC microarray devices.