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NNC55-0396 Reduces SH-SY5Y Cell Damage Induced by Bupivacaine Hydrochloride
- Source :
- Journal of Experimental and Clinical Toxicology. 1:17-29
- Publication Year :
- 2020
- Publisher :
- Open Access Pub, 2020.
-
Abstract
- Background Local anesthetic neurotoxicity is a common complication in clinical anesthesia, which can cause permanent nerve damage in severe cases. The T-type calcium channel is an important channel for regulating the excitability of neurons. Normally, extracellular calcium ions enter the cell through the T-type calcium channel to change the excitability of neurons. When the intracellular calcium is overloaded, it can cause cell damage. Aims To investigated the roles of T-type calcium channel in the SH-SY5Y cells injury induced by the bupivacaine. Methods The SH-SY5Y cell culture model was used to observe the effect of T-type calcium channel blocker NNC55-0396 on the neurotoxicity of bupivacaine hydrochloride by MTT methold,flow cytometry, Western blotting and other methods. Results The results show that NNC55-0396 can block the T-type calcium channel of SH-SY5Y cells, improve the decrease of cell viability caused by bupivacaine hydrochloride, reduce the level of intracellular calcium ion, reduce the expression of Cleavedcaspase-3, and reduce cell apoptosis. Conclusion The above results indicate that the T-type calcium channel is involved in the SH-SY5Y cell damage caused by bupivacaine hydrochloride, and blocking the T-type calcium channel can reduce the neurotoxicity of bupivacaine hydrochloride.
Details
- ISSN :
- 26417669
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental and Clinical Toxicology
- Accession number :
- edsair.doi...........11a4f4dfb182318cd163e2248f99bc09