The Effectiveness of Chloramphenicol In-Situ Opthalmic Gel with Base Poloxamer 407 and HPMC Against Staphylococcus Aureus Atcc 29213 And Pseudomonas Aeruginosa Atcc 27853

Introduction: In-situ gel is a simple liquid transparent polymer solution under storage conditions, but turns into a viscoelastic gel after entering the eye due to the phase transition properties of the polymer that increase the residence time in ocular organ and bioavailability, enabling the delivery of reproducible doses and improving patient compliance. The aim of the present study was to formulate and evaluate the antibacterial effectivity of chloramphenicol in-situ ophthalmic gel with base poloxamer 407 and HPMC base against Staphylococcus aureus and Pseudomonas aeruginosa. Material and Methods: The optimization of ophthalmic gel preparation by the factorial design method has been carried out in order to know the best formula of all the formulas employed with 0.5% chloramphenicol active substance, wherein each formula was obtained from high concentration and low concentration of each base. Results: The measurement of the antibacterial effectivity against Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853 by oneway ANOVA analysis showed that formula with base poloxamer 407 5% (F1) gave the best result. F1 has a dilute consistency, clear and stable during 28 days storage time when effectiveness test performed. Conclusions: Chloramphenicol in-situ gel with base poloxamer 407 and HPMC were effective against Staphylococcus aureus ATCC 29213 with intermediate to sensitive category, and Pseudomonas aeruginosa ATCC 27853 with sensitive category in accordance to the requirements of the Clinical and Laboratory Standards Institute (CLSI).