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13C-TmDOTA as versatile thermometer compound for solid-state NMR of hydrated lipid bilayer membranes
- Source :
- Magnetic Resonance in Chemistry. 54:227-233
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Recent advances in solid-state nuclear magnetic resonance (NMR) techniques, such as magic angle spinning and high-power decoupling, have dramatically increased the sensitivity and resolution of NMR. However, these NMR techniques generate extra heat, causing a temperature difference between the sample in the rotor and the variable temperature gas. This extra heating is a particularly crucial problem for hydrated lipid membrane samples. Thus, to develop an NMR thermometer that is suitable for hydrated lipid samples, thulium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (TmDOTA) was synthesized and labeled with (13) C (i.e., (13) C-TmDOTA) to increase the NMR sensitivity. The complex was mixed with a hydrated lipid membrane, and the system was subjected to solid-state NMR and differential scanning calorimetric analyses. The physical properties of the lipid bilayer and the quality of the NMR spectra of the membrane were negligibly affected by the presence of (13) C-TmDOTA, and the (13) C chemical shift of the complex exhibited a large-temperature dependence. The results demonstrated that (13) C-TmDOTA could be successfully used as a thermometer to accurately monitor temperature changes induced by (1) H decoupling pulses and/or by magic angle spinning and the temperature distribution of the sample inside the rotor. Thus, (13) C-TmDOTA was shown to be a versatile thermometer for hydrated lipid assemblies. Copyright © 2015 John Wiley & Sons, Ltd.
- Subjects :
- Chemistry
Resolution (electron density)
Analytical chemistry
General Chemistry
010402 general chemistry
01 natural sciences
Temperature measurement
030218 nuclear medicine & medical imaging
0104 chemical sciences
NMR spectra database
03 medical and health sciences
0302 clinical medicine
Membrane
Solid-state nuclear magnetic resonance
Thermometer
Magic angle spinning
General Materials Science
Lipid bilayer
Subjects
Details
- ISSN :
- 07491581
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Magnetic Resonance in Chemistry
- Accession number :
- edsair.doi...........101fafd92506a6114ee4a1bfdcce5534