In Vitro Properties of MEDI-4212, a Human Anti-IgE Antibody for the Treatment of Allergic Asthma

Authors :: Mats Carlsson
S Klakamp
Dorothy A. Sims
Sarah Oakley
Ian A. Anderson
S OBrian
Chris Lloyd
Claire Dobson
Sofia Persdotter
M Azimi
Per-Olof Fredrik Eriksson
Anthony J. Coyle
U Alkner
K von Wachenfeldt
Phillip Monk
I de Mendez
H. Ekdahl
E. S. Cohen
Source :: D33. ASTHMA THERAPEUTICS -- STUDIES IN ANIMAL MODELS.
Publication Year :: 2009
Publisher :: American Thoracic Society, 2009.
Abstract: HuFceRI transfected RBL or LAD2 cells were incubated with IgE ± MEDI4212. Bound IgE was cross-linked using a commercial anti-IgE and cell activation detected using calcium signalling or β-hexosaminidase release. Effect of MEDI4212 on IgE/CD23 interaction was assessed by incubation of IL-4 stimulated IM9 cells with IgE ± MEDI4212 and bound IgE detected by flow cytometry. Effects on IgE production in human B cells (differentiated with APC + IL-4, ± MEDI4212) was evaluated by flow cytometry. Serum free-IgE was measured using ELISA.

Subjects :: biology
medicine.diagnostic_test
Chemistry
CD23
Transfection
Immunoglobulin E
Molecular biology
In vitro
Flow cytometry
biology.protein
medicine
Cell activation
Incubation
Calcium signaling

Database :: OpenAIRE
Journal :: D33. ASTHMA THERAPEUTICS -- STUDIES IN ANIMAL MODELS
Accession number :: edsair.doi...........0f76b7a78a12c924dad35873a7214081
Full Text :: https://doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5726

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