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The effects of mesocarbon microbeads support for platinum–ruthenium (Pt–Ru) catalysts on anode performance of the direct methanol fuel cell (DMFC) were investigated. Polarization characteristics of the anode electrode were low due to the fast rate of mass transport in the electrode. The effects of the Nafion® content in the catalyst, the MEA hot press condition, the cell operation temperature and methanol concentration on the polarization curves of the anode were also investigated.